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protein subcellular localization analysis  (Bio-Rad)


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    Bio-Rad protein subcellular localization analysis
    Protein Subcellular Localization Analysis, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 44931 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/protein+subcellular+localization+analysis/Protein+Standard+I/pmc12167944__pnas%2E2415835122%2Esapp-13-1-34
    Average 99 stars, based on 44931 article reviews
    protein subcellular localization analysis - by Bioz Stars, 2026-09
    99/100 stars

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    Sequencing:

    Article Title: Stepwise deactivation of gibberellins during rice internode elongation
    Article Snippet: .. For protein subcellular localization analysis, the coding sequence of EUI2 was amplified and inserted into pA7-YFP, giving rise to pA7-EUI2-YFP, which was transformed into onion epidermal cells was performed using a helium biolistic device (Bio-Rad PDS-1000). .. The samples were observed with a confocal laser microscope (Zeiss LSM510).

    Amplification:

    Article Title: Stepwise deactivation of gibberellins during rice internode elongation
    Article Snippet: .. For protein subcellular localization analysis, the coding sequence of EUI2 was amplified and inserted into pA7-YFP, giving rise to pA7-EUI2-YFP, which was transformed into onion epidermal cells was performed using a helium biolistic device (Bio-Rad PDS-1000). .. The samples were observed with a confocal laser microscope (Zeiss LSM510).

    Transformation Assay:

    Article Title: Stepwise deactivation of gibberellins during rice internode elongation
    Article Snippet: .. For protein subcellular localization analysis, the coding sequence of EUI2 was amplified and inserted into pA7-YFP, giving rise to pA7-EUI2-YFP, which was transformed into onion epidermal cells was performed using a helium biolistic device (Bio-Rad PDS-1000). .. The samples were observed with a confocal laser microscope (Zeiss LSM510).



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    Image Search Results


    The characteristics of 57 C2H2 proteins identified and their subcellular localization prediction.

    Journal: International Journal of Molecular Sciences

    Article Title: Transcriptomic Identification of Potential C2H2 Zinc Finger Protein Transcription Factors in Pinus massoniana in Response to Biotic and Abiotic Stresses

    doi: 10.3390/ijms25158361

    Figure Lengend Snippet: The characteristics of 57 C2H2 proteins identified and their subcellular localization prediction.

    Article Snippet: Subcellular localization prediction and analysis of PmC2H2 protein were conducted using CELLO ( http://cello.life.nctu.edu.tw/ ) (accessed on 3 August 2023), WoLF PSORT ( https://wolfpsort.hgc.jp/ ) (accessed on 3 August 2023), and Plant-mPLoc tools ( http://www.csbio.sjtu.edu.cn/bioinf/plant-multi/ ) (accessed on 3 August 2023).

    Techniques:

    Phylogenetic analysis and motif distribution of PmC2H2 ZFPs. Branches with different colors represented different subgroups. The subgroup was named from “I” to “V”.

    Journal: International Journal of Molecular Sciences

    Article Title: Transcriptomic Identification of Potential C2H2 Zinc Finger Protein Transcription Factors in Pinus massoniana in Response to Biotic and Abiotic Stresses

    doi: 10.3390/ijms25158361

    Figure Lengend Snippet: Phylogenetic analysis and motif distribution of PmC2H2 ZFPs. Branches with different colors represented different subgroups. The subgroup was named from “I” to “V”.

    Article Snippet: Subcellular localization prediction and analysis of PmC2H2 protein were conducted using CELLO ( http://cello.life.nctu.edu.tw/ ) (accessed on 3 August 2023), WoLF PSORT ( https://wolfpsort.hgc.jp/ ) (accessed on 3 August 2023), and Plant-mPLoc tools ( http://www.csbio.sjtu.edu.cn/bioinf/plant-multi/ ) (accessed on 3 August 2023).

    Techniques:

    Sequences of the 10 motifs of PmC2H2 ZFPs.

    Journal: International Journal of Molecular Sciences

    Article Title: Transcriptomic Identification of Potential C2H2 Zinc Finger Protein Transcription Factors in Pinus massoniana in Response to Biotic and Abiotic Stresses

    doi: 10.3390/ijms25158361

    Figure Lengend Snippet: Sequences of the 10 motifs of PmC2H2 ZFPs.

    Article Snippet: Subcellular localization prediction and analysis of PmC2H2 protein were conducted using CELLO ( http://cello.life.nctu.edu.tw/ ) (accessed on 3 August 2023), WoLF PSORT ( https://wolfpsort.hgc.jp/ ) (accessed on 3 August 2023), and Plant-mPLoc tools ( http://www.csbio.sjtu.edu.cn/bioinf/plant-multi/ ) (accessed on 3 August 2023).

    Techniques:

    Subcellular localization analysis of PmC2H2-4 and PmC2H2-20 proteins in N. benthamiana leaves. The scale in the images is 20 μm. pCAMBIA-1302-mGFP5 was the control. DAPI—4′,6-diamidino-2-phenylindole, a blue fluorescent dye that shows DNA location. Chloroplast—chloroplast auto-fluorescence, displays the location of chloroplasts. GFP—green fluorescence protein, displays the location of the target protein. Bright—bright field. Merged—merged picture of four overlapped channels.

    Journal: International Journal of Molecular Sciences

    Article Title: Transcriptomic Identification of Potential C2H2 Zinc Finger Protein Transcription Factors in Pinus massoniana in Response to Biotic and Abiotic Stresses

    doi: 10.3390/ijms25158361

    Figure Lengend Snippet: Subcellular localization analysis of PmC2H2-4 and PmC2H2-20 proteins in N. benthamiana leaves. The scale in the images is 20 μm. pCAMBIA-1302-mGFP5 was the control. DAPI—4′,6-diamidino-2-phenylindole, a blue fluorescent dye that shows DNA location. Chloroplast—chloroplast auto-fluorescence, displays the location of chloroplasts. GFP—green fluorescence protein, displays the location of the target protein. Bright—bright field. Merged—merged picture of four overlapped channels.

    Article Snippet: Subcellular localization prediction and analysis of PmC2H2 protein were conducted using CELLO ( http://cello.life.nctu.edu.tw/ ) (accessed on 3 August 2023), WoLF PSORT ( https://wolfpsort.hgc.jp/ ) (accessed on 3 August 2023), and Plant-mPLoc tools ( http://www.csbio.sjtu.edu.cn/bioinf/plant-multi/ ) (accessed on 3 August 2023).

    Techniques: Control, Fluorescence

    The figure above shows the expression levels of five PmC2H2 genes under different abiotic treatments, namely ( a ) ABA, ( b ) drought, ( c ) ETH, ( d ) H 2 O 2 , ( e ) mechanical damage, ( f ) MeJA, ( g ) NaCl, ( h ) PEG, and ( i ) SA. The absence of any significant difference is indicated by the presence of identical lowercase letters across different columns. Conversely, the presence of completely distinct lowercase letters between different columns signifies a statistically significant difference ( p < 0.05). In cases where multiple lowercase letters are present within the same column, it implies that there is no significant difference between that particular column and other columns containing any one of those lowercase letters. The relative expression at 0 h is normalized to “1”.

    Journal: International Journal of Molecular Sciences

    Article Title: Transcriptomic Identification of Potential C2H2 Zinc Finger Protein Transcription Factors in Pinus massoniana in Response to Biotic and Abiotic Stresses

    doi: 10.3390/ijms25158361

    Figure Lengend Snippet: The figure above shows the expression levels of five PmC2H2 genes under different abiotic treatments, namely ( a ) ABA, ( b ) drought, ( c ) ETH, ( d ) H 2 O 2 , ( e ) mechanical damage, ( f ) MeJA, ( g ) NaCl, ( h ) PEG, and ( i ) SA. The absence of any significant difference is indicated by the presence of identical lowercase letters across different columns. Conversely, the presence of completely distinct lowercase letters between different columns signifies a statistically significant difference ( p < 0.05). In cases where multiple lowercase letters are present within the same column, it implies that there is no significant difference between that particular column and other columns containing any one of those lowercase letters. The relative expression at 0 h is normalized to “1”.

    Article Snippet: Subcellular localization prediction and analysis of PmC2H2 protein were conducted using CELLO ( http://cello.life.nctu.edu.tw/ ) (accessed on 3 August 2023), WoLF PSORT ( https://wolfpsort.hgc.jp/ ) (accessed on 3 August 2023), and Plant-mPLoc tools ( http://www.csbio.sjtu.edu.cn/bioinf/plant-multi/ ) (accessed on 3 August 2023).

    Techniques: Expressing

    Transcriptional activation assay of six PmC2H2 genes. Empty pGBKT7 vector was used as a negative control and pGBKT7- PmC3H20 was used as a positive control.

    Journal: International Journal of Molecular Sciences

    Article Title: Transcriptomic Identification of Potential C2H2 Zinc Finger Protein Transcription Factors in Pinus massoniana in Response to Biotic and Abiotic Stresses

    doi: 10.3390/ijms25158361

    Figure Lengend Snippet: Transcriptional activation assay of six PmC2H2 genes. Empty pGBKT7 vector was used as a negative control and pGBKT7- PmC3H20 was used as a positive control.

    Article Snippet: Subcellular localization prediction and analysis of PmC2H2 protein were conducted using CELLO ( http://cello.life.nctu.edu.tw/ ) (accessed on 3 August 2023), WoLF PSORT ( https://wolfpsort.hgc.jp/ ) (accessed on 3 August 2023), and Plant-mPLoc tools ( http://www.csbio.sjtu.edu.cn/bioinf/plant-multi/ ) (accessed on 3 August 2023).

    Techniques: Activation Assay, Plasmid Preparation, Negative Control, Positive Control